HOMER1 Monoklonaler Antikörper

HOMER1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B1B11

Kat-Nr. : 68597-1-Ig

Synonyme

3B1B11, HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1

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  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 68597-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using HOMER1 antibody (68597-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Hühnerhirngewebe, HEK-293-Zellen, LNCaP-Zellen, MOLT-4-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, Hausschwein-Cerebellum-Gewebe, Kaninchenhirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68597-1-Ig bindet in WB, IF/ICC, ELISA HOMER1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HOMER1 fusion protein Ag21302
• Vollständiger Name: homer homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-46 kDa
• GenBank-Zugangsnummer: BC015502
• Gene symbol: HOMER1
• Gene ID (NCBI): 9456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.