HOMER1 Monoklonaler Antikörper

HOMER1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B1B11

Kat-Nr. : 68597-1-PBS

Synonyme

3B1B11, HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1

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  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 68597-1-Ig (same clone as 68597-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using HOMER1 antibody (68597-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68597-1-PBS bindet in WB, IF/ICC, Indirect ELISA HOMER1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HOMER1 fusion protein Ag21302
• Vollständiger Name: homer homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-46 kDa
• GenBank-Zugangsnummer: BC015502
• Gene symbol: HOMER1
• Gene ID (NCBI): 9456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.