HOMER1 Rekombinanter Antikörper

HOMER1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243007G2

Kat-Nr. : 85692-4-PBS

Synonyme

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

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  WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IF Staining of MOLT-4 using 85692-4-RR (same clone as 85692-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using HOMER1 antibody (85692-4-RR, Clone: 243007G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85692-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

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Geprüfte Anwendungen

Produktinformation

85692-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HOMER1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HOMER1 fusion protein Ag3109
• Vollständiger Name: homer homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC015502
• Gene symbol: HOMER1
• Gene ID (NCBI): 9456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.