HOOK1 Polyklonaler Antikörper

HOOK1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10871-1-PBS

Synonyme

Protein Hook homolog 1, hook homolog 1 (Drosophila), HK1, h-hook1, hHK1

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  WB analysis of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10871-1-AP (same clone as 10871-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10871-1-AP (same clone as 10871-1-PBS)

  IP result of anti-HOOK1 (IP:10871-1-AP, 4ug; Detection:10871-1-AP 1:500) with mouse brain tissue lysate 4680ug. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10871-1-AP (same clone as 10871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10871-1-AP (HOOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10871-1-AP (HOOK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HOOK1 antibody (10871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10871-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HOOK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOOK1 fusion protein Ag1311
• Vollständiger Name: hook homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC011621
• Gene symbol: HOOK1
• Gene ID (NCBI): 51361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.