HOOK3 Polyklonaler Antikörper

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• KD/KO Validated

HOOK3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15457-1-PBS

Synonyme

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• 

  WB analysis of rat liver using 15457-1-AP (same clone as 15457-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 15457-1-AP (same clone as 15457-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 15457-1-AP (same clone as 15457-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 15457-1-AP (same clone as 15457-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 15457-1-AP (same clone as 15457-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 15457-1-AP (same clone as 15457-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 15457-1-AP (same clone as 15457-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 15457-1-AP (same clone as 15457-1-PBS)

  IP result of anti-HOOK3 (IP:15457-1-AP, 4ug; Detection:15457-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15457-1-AP (same clone as 15457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15457-1-AP (same clone as 15457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15457-1-AP (same clone as 15457-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15457-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HOOK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOOK3 fusion protein Ag7724
• Vollständiger Name: hook homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC056146
• Gene symbol: HOOK3
• Gene ID (NCBI): 84376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.