PSMC3IP Polyklonaler Antikörper

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• KD/KO Validated

PSMC3IP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11339-1-PBS

Synonyme

PSMC3-interacting protein, PSMC3 interacting protein, Proteasome 26S ATPase subunit 3-interacting protein, HOP2, Homologous-pairing protein 2 homolog

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  WB analysis using 11339-1-AP (same clone as 11339-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11339-1-AP (same clone as 11339-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11339-1-AP (same clone as 11339-1-PBS)

  IP result of anti-HOP2 (IP:11339-1-AP, 4ug; Detection:11339-1-AP 1:500) with Jurkat cells lysate 3200ug. This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11339-1-AP (same clone as 11339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11339-1-AP (same clone as 11339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 11339-1-AP (same clone as 11339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 11339-1-AP (same clone as 11339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11339-1-AP (same clone as 11339-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOP2 antibody (11339-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11339-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11339-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PSMC3IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMC3IP fusion protein Ag1883
• Vollständiger Name: PSMC3 interacting protein
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 24-29 kDa
• GenBank-Zugangsnummer: BC008792
• Gene symbol: PSMC3IP
• Gene ID (NCBI): 29893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMC3IP, also called HOP2, encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis.