HOPX Polyklonaler Antikörper

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HOPX Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11419-1-PBS

Synonyme

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  WB analysis using 11419-1-AP (same clone as 11419-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 11419-1-AP (same clone as 11419-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 11419-1-AP (same clone as 11419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 11419-1-AP (same clone as 11419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11419-1-AP (same clone as 11419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11419-1-AP (same clone as 11419-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11419-1-PBS bindet in WB, IHC, Indirect ELISA HOPX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOPX fusion protein Ag1979
• Vollständiger Name: HOP homeobox
• Berechnetes Molekulargewicht: 73 aa, 8 kDa
• Beobachtetes Molekulargewicht: 8-12 kDa
• GenBank-Zugangsnummer: BC014225
• Gene symbol: HOPX
• Gene ID (NCBI): 84525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOPX (Homeodomain-only protein) gene has various synonyms including HOD, HOP, OB1, LAGY and NECC1. The protein encoded by this gene is an unusual homeodomain protein that lacks certain conserved residues required for DNA binding. HOPX has diverse effects on cardiac growth. Manipulation of Hopx function in murine models is associated with cardiac hypertrophy, dilation and fibrosis. HOPX protein acts as an antagonist to the serum response factor (SRF), which regulates the opposing processes of cell proliferation and differentiation. Overexpression of HOPX causes cardiac hypertrophy. HOPX protein can inhibit SRF-dependent transcriptional activation by recruiting histone deacetylase (HDAC) activity.