HORMAD1 Monoklonaler Antikörper

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HORMAD1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D7E12

Kat-Nr. : 67091-1-PBS

Synonyme

2D7E12, CT46, HORMA domain-containing protein 1, HORMAD 1, NOHMA

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  WB analysis of human testis using 67091-1-Ig (same clone as 67091-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67091-1-Ig (same clone as 67091-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67091-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HORMAD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HORMAD1 fusion protein Ag28316
• Vollständiger Name: HORMA domain containing 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC047406
• Gene symbol: HORMAD1
• Gene ID (NCBI): 84072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].