HOXA7 Monoklonaler Antikörper

HOXA7 Monoklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2A7H6

Kat-Nr. : 67112-1-Ig

Synonyme

ANTP, homeobox A7, Homeobox protein Hox 1.1, Homeobox protein Hox 1A, Homeobox protein Hox A7, HOX1, HOX1.1, HOX1A, HOXA7

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Galerie der Validierungsdaten

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  WB analysis of THP-1 using 67112-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67112-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 67112-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67112-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67112-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67112-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67112-1-Ig (HOXA7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HepG2-Zellen, HL-60-Zellen, HT-29-Zellen, K-562-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67112-1-Ig bindet in WB, IHC, IF, ELISA HOXA7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HOXA7 fusion protein Ag19025
• Vollständiger Name: homeobox A7
• Berechnetes Molekulargewicht: 230 aa, 25 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC148692
• Gene symbol: HOXA7
• Gene ID (NCBI): 3204
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOX genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene Hoxa-1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor, which has been shown to play important roles in cell differentiation and embryogenesis. Hoxa-1 is also expressed in cancers, such as mammary tumors, though it is not expressed in normal gland or in precancerous mammary tissues. At embryonic stages, Hoxa-2 is expressed in the mesenchyme and epithelial cells of palate, however its expression is restricted to the tips of the growing palatal shelves. Hoxa-2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Hoxa-7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. Hoxa-7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta.

Publikationen

Species	Application	Title
human	WB	Reprod Sci; CircPAPPA Regulates the Proliferation, Migration, Invasion, Apoptosis, and Cell Cycle of Trophoblast Cells Through the miR-3127-5p/HOXA7 Axis.; Authors - Jun Li
human	WB	J Orthop Surg Res; MiR-920 promotes osteogenic differentiation of human bone mesenchymal stem cells by targeting HOXA7.; Authors - Jun-Pu Zha
human,mouse	WB,IHC,IF	Cancer Sci; Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment; Authors - Anqi Feng ; KD Validated