HOXB7 Polyklonaler Antikörper

HOXB7 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12613-1-AP

Synonyme

HHO.C1, Homeobox protein HHO.C1, Homeobox protein Hox-2C, Homeobox protein Hox-B7, HOX2C

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:300.

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  IF Staining of HepG2 using 12613-1-AP

  Immunofluorescent analysis of HepG2 cells using 12613-1-AP (HOXB7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen
• Erfolgreiche IP: SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

12613-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HOXB7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOXB7 fusion protein Ag3245
• Vollständiger Name: homeobox B7
• Berechnetes Molekulargewicht: 217 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-30 kDa
• GenBank-Zugangsnummer: BC015345
• Gene symbol: HOXB7
• Gene ID (NCBI): 3217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOXB7 is a member of the homeobox family genes, which are often dysregulated in various cancer types. Particularly HOXB7 amplification and overexpression correlate with poor prognosis in various cancer such as gastric, pancreatic, and lung cancers. Moreover, HOXB7 is known to contribute to cancer progression by promoting epithelial to mesenchymal transition, anticancer drug resistance, and angiogenesis (PMID: 36659836).

Publikationen

Species	Application	Title
rat	WB	J Neuroinflammation; Gene expression comparison reveals distinct basal expression of HOX members and differential TNF-induced response between brain- and spinal cord-derived microvascular endothelial cells.; Authors - Yves Molino
human	WB	Int J Oncol; CircRNA hsa_circ_0070934 functions as a competitive endogenous RNA to regulate HOXB7 expression by sponging miR‑1236‑3p in cutaneous squamous cell carcinoma.; Authors - Da-Wei Zhang
human	WB	Exp Cell Res; Long non-coding RNA BBOX1-AS1 exacerbates esophageal squamous cell carcinoma development by regulating HOXB7/β-catenin axis.; Authors - Jinxiu Sheng
human	WB,IHC	Biomed Res Int; Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer; Authors - Jialei Chen