HOXB9 Rekombinanter Antikörper

HOXB9 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241866C6

Kat-Nr. : 84609-3-RR

Synonyme

HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5

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  WB analysis of K-562 using 84609-3-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84609-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84609-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84609-3-RR against Human HOXB9 were performed. The affinity constant is 40.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HCT 116-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84609-3-RR bindet in WB, ELISA HOXB9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HOXB9 fusion protein Ag34837
• Vollständiger Name: homeobox B9
• Berechnetes Molekulargewicht: 250 aa, 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC015565
• Gene symbol: HOXB9
• Gene ID (NCBI): 3219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOXB9 is a member of the Homeobox (HOX) gene family, which plays a crucial role in embryonic development and is involved in the regulation of gene expression during this process. In addition to its role in embryogenesis, HOXB9 has been implicated in various cancers, including breast and prostate cancer. It is overexpressed in breast cancer tissue and is regulated by estrogen, playing a critical role in angiogenesis. HOXB9 overexpression has been shown to stimulate three-dimensional colony formation in soft-agar assays, indicating its role in tumorigenesis. Furthermore, HOXB9 binds to the promoters of various tumor growth and angiogenic factors, regulating their expression, and its homeodomain plays a crucial role in transcriptional regulation and tumorigenesis.