HOXC4 Polyklonaler Antikörper

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• KD/KO Validated

HOXC4 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14321-1-AP

Synonyme

cp19, homeobox C4, Homeobox protein CP19, Homeobox protein Hox 3E, Homeobox protein Hox C4, HOX3, HOX3E, HOXC4

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Galerie der Validierungsdaten

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  WB analysis using 14321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14321-1-AP (HOXC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14321-1-AP

  WB result of HOXC4 antibody (14321-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HOXC4 transfected Jurkat cells.

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  IF Staining of HeLa using 14321-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14321-1-AP (HOXC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, PC-12-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14321-1-AP bindet in WB, IF, ELISA HOXC4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOXC4 fusion protein Ag5563
• Vollständiger Name: homeobox C4
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC050442
• Gene symbol: HOXC4
• Gene ID (NCBI): 3221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The class I homeobox (HOX) family of homeodomain-containing transcription factors has 39 members in mammals. These genes are distributed throughout the genome in four clusters: HOXA (7p15), HOXB (17q21), HOXC (12q13), and HOXD (2q31). HOX genes are known to act as transcription factors, playing an essential regulatory role in the temporal and spatial development of the embryo. HOXC family members are not expressed in normal prostate tissue but increased expression of HOXC genes is commonly detected in prostate cancers. HOXC4 is expressed in GC B cells and HoxC4 expression is specifically up-regulated by the same stimuli that induce AID expression (LPS, CD154 and IL-4). The calculated molecular weight of HOXC4 is 30 kDa. Sometimes higher molecular weight around 39 kDa can also be observed, which may be a modified variant of HOXC4 (PMID: 23178148, 32012197, 35400027).

Publikationen

Species	Application	Title
human	WB	Oncotarget; Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.; Authors - Chao Liu