HP1BP3 Polyklonaler Antikörper

HP1BP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24556-1-AP

Synonyme

Protein HP1-BP74, Protein HP1 BP74, HP1 BP74, Heterochromatin protein 1-binding protein 3

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  WB analysis using 24556-1-AP

  Mouse thymus lysates were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 24556-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24556-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24556-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24556-1-AP

  IP result of anti-HP1BP3 (IP:24556-1-AP, 4ug; Detection:24556-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human skin cancer using 24556-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 24556-1-AP (HP1BP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HP1BP3 antibody (24556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

24556-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HP1BP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HP1BP3 fusion protein Ag18709
• Vollständiger Name: heterochromatin protein 1, binding protein 3
• Berechnetes Molekulargewicht: 553 aa, 61 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC045660
• Gene symbol: HP1BP3
• Gene ID (NCBI): 50809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.

Publikationen

Species	Application	Title
human	WB	Neurobiol Aging; Systems genetics identifies Hp1bp3 as a novel modulator of cognitive aging.; Authors - Sarah M Neuner
mouse	WB,IF	Sci Adv; Cpeb1 remodels cell type-specific translational program to promote fear extinction; Authors - Juan Zhang