HP1BP3 Polyklonaler Antikörper

HP1BP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24556-1-PBS

Synonyme

Protein HP1-BP74, Protein HP1 BP74, HP1 BP74, Heterochromatin protein 1-binding protein 3

---

• 

  WB analysis using 24556-1-AP (same clone as 24556-1-PBS)

  Mouse thymus lysates were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 24556-1-AP (same clone as 24556-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 24556-1-AP (same clone as 24556-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

  IP result of anti-HP1BP3 (IP:24556-1-AP, 4ug; Detection:24556-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 24556-1-AP (same clone as 24556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 24556-1-AP (HP1BP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HP1BP3 antibody (24556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

24556-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HP1BP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HP1BP3 fusion protein Ag18709
• Vollständiger Name: heterochromatin protein 1, binding protein 3
• Berechnetes Molekulargewicht: 553 aa, 61 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC045660
• Gene symbol: HP1BP3
• Gene ID (NCBI): 50809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.