HPRT Monoklonaler Antikörper

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• KD/KO Validated

HPRT Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C3D1

Kat-Nr. : 67518-1-PBS

Synonyme

HPRT1, 1C3D1, EC:2.4.2.8, HGPRT, HGPRTase

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  WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT deficient cell line Sp2/0 was served as a negative control. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

  WB result of HPRT antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT transfected HEK-293 cells. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HPRT antibody (67518-1-Ig, Clone: 1C3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67518-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HPRT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HPRT fusion protein Ag7044
• Vollständiger Name: hypoxanthine phosphoribosyltransferase 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC000578
• Gene symbol: HPRT1
• Gene ID (NCBI): 3251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)