HPS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HPS1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, RIP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15077-1-AP

Synonyme

Hermansky Pudlak syndrome 1, HPS, HPS1

---

Galerie der Validierungsdaten

• 

  WB analysis of L02 using 15077-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15077-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15077-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 15077-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15077-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of L02 using 15077-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 15077-1-AP (HPS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 15077-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HPS1 antibody (15077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, K-562-Zellen, L02-Zellen, Mauslungengewebe, Raji-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF: L02-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

15077-1-AP bindet in WB, RIP, IF, ELISA HPS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HPS1 fusion protein Ag7149
• Vollständiger Name: Hermansky-Pudlak syndrome 1
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 70-79 kDa
• GenBank-Zugangsnummer: BC000175
• Gene symbol: HPS1
• Gene ID (NCBI): 3257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HPS1, also named as Hermansky-Pudlak syndrome 1 protein, is a 700 amino acid protein, which is a component of multiple cytoplasmic organelles. HPS1 is Apparently crucial for their normal development and function and involved in intracellular protein sorting.HPS1 is involved in the biogenesis of early melanosomes.

Publikationen

Species	Application	Title
mouse	WB,IF	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook ; KO Validated
		Am J Respir Cell Mol Biol; Dysregulation of Galectin-3: Implications for Hermansky-Pudlak Syndrome Pulmonary Fibrosis.; Authors - Cullinane Andrew R AR
human	RIP	Clin Exp Metastasis; Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1.; Authors - Tongxin Xu
human	WB	Mol Genet Metab; In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.; Authors - Yasuhiro Ikawa