HPSE Monoklonaler Antikörper

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• KD/KO Validated

HPSE Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D8B8

Kat-Nr. : 66226-1-PBS

Synonyme

1D8B8, EC:3.2.1.166, Endo glucoronidase, Endo-glucoronidase, HEP

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  WB analysis of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66226-1-Ig (HPSE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66226-1-Ig(HPSE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66226-1-PBS bindet in WB, IHC, IF/ICC, ELISA HPSE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HPSE fusion protein Ag10067
• Vollständiger Name: heparanase
• Berechnetes Molekulargewicht: 543 aa, 61 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC051321
• Gene symbol: HPSE
• Gene ID (NCBI): 10855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.