HRAS Polyklonaler Antikörper

HRAS Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 15531-1-AP

Synonyme

GTPase HRas, H RASIDX, HRAS1, C BAS/HAS, C H RAS

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  WB analysis using 15531-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 15531-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15531-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15531-1-AP

  IP result of anti-HRAS (IP:15531-1-AP, 4ug; Detection:15531-1-AP 1:300) with HeLa cells lysate 2600 ug.

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  IHC staining of human stomach using 15531-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15531-1-AP (HRAS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse intestine using 15531-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 15531-1-AP (HRAS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Mausnierengewebe, PC-12-Zellen, Rattennierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, mouse intestine; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15531-1-AP bindet in WB, IHC, IP, ELISA HRAS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HRAS fusion protein Ag7704
• Vollständiger Name: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC006499
• Gene symbol: HRAS
• Gene ID (NCBI): 3265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HRAS belongs to the small GTPase superfamily and Ras family. RAS family proteins (KRAS4A, KRAS4B, NRAS and HRAS) function as GDP-GTP-regulated binary on-off switches, which regulate cytoplasmic signaling networks that control diverse normal cellular processes (PMID: 26985062). HRAS is Involved in the activation of Ras protein signal transduction (PMID: 22821884). As HRAS mutations are associated to activation of RAF/MEK/ERK signaling in different cancers (PMID: 25435214). HRAS is mostly altered in head and neck squamous cell carcinoma (HNSCC) (5-9%), salivary glands (15%), and bladder cancer (5-30%) (PMID: 35621690).

Publikationen

Species	Application	Title
human	WB	Mol Med Rep; Sirtuin 2 knockdown inhibits cell proliferation and RAS/ERK signaling, and promotes cell apoptosis and cell cycle arrest in multiple myeloma.; Authors - Tianling Ding
human	WB	Naunyn Schmiedebergs Arch Pharmacol; The potential mechanism of Neu5Gc inducing colorectal cancer based on network pharmacology and experimental validation; Authors - Li-Chao Zhang
human	WB	Oncogene; Inhibition and degradation of NRAS with a pan-NRAS monobody; Authors - Michael Whaby