HRC Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HRC Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 18142-1-AP

Synonyme

Sarcoplasmic reticulum histidine-rich calcium-binding protein

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  WB analysis of mouse heart using 18142-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 18142-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 18142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 18142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18142-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

18142-1-AP bindet in WB, IHC, IF/ICC, ELISA HRC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HRC fusion protein Ag12794
• Vollständiger Name: histidine rich calcium binding protein
• Berechnetes Molekulargewicht: 699 aa, 80 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC069795
• Gene symbol: HRC
• Gene ID (NCBI): 3270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
Human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
mouse	IF	Elife; Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy; Authors - Wei Li
mouse	WB	Chem Biol Interact; Vitamin D alleviates liver fibrosis by inhibiting histidine-rich calcium binding protein (HRC).; Authors - Wanyi Lu
human	WB,IHC	J Adv Res; Inhibition of lung cancer by vitamin D depends on downregulation of histidine-rich calcium-binding protein.; Authors - Ning Liu ; KO Validated