HRC Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HRC Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18142-1-PBS

Synonyme

---

• 

  WB analysis of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 18142-1-AP (same clone as 18142-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

18142-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HRC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HRC fusion protein Ag12794
• Vollständiger Name: histidine rich calcium binding protein
• Berechnetes Molekulargewicht: 699 aa, 80 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC069795
• Gene symbol: HRC
• Gene ID (NCBI): 3270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.