HRD1/SYVN1 Monoklonaler Antikörper

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• KD/KO Validated

HRD1/SYVN1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C8A7

Kat-Nr. : 67488-1-PBS

Synonyme

SYVN1, 2C8A7, EC:2.3.2.27, HRD1, KIAA1810

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  WB analysis using 67488-1-Ig (same clone as 67488-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67488-1-Ig (same clone as 67488-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67488-1-Ig (same clone as 67488-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

  WB result of HRD1/SYVN1 antibody (67488-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRD1/SYVN1 antibody (67488-1-Ig, Clone: 2C8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67488-1-PBS bindet in WB, IHC, IF/ICC, ELISA HRD1/SYVN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HRD1/SYVN1 fusion protein Ag4456
• Vollständiger Name: synovial apoptosis inhibitor 1, synoviolin
• Berechnetes Molekulargewicht: 617 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68-76 kDa
• GenBank-Zugangsnummer: BC030530
• Gene symbol: HRD1
• Gene ID (NCBI): 84447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).