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HRH1 Polyklonaler Antikörper

HRH1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28763-1-AP

Synonyme

H1 R, H1R, HH1R, hisH1, Histamine H1 receptor

Filter:
  • Western Blot (WB) analysis of various lysates using HRH1 Polyclonal antibody (28763-1-AP)

    WB analysis using 28763-1-AP

    MCF-7 cells lysates were subjected to SDS PAGE followed by western blot with 28763-1-AP (HRH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using HRH1 Polyclonal antibody (28763-1-AP)

    WB analysis of rat brain using 28763-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 28763-1-AP (HRH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HRH1 Polyclonal antibody (28763-1-AP)

    IHC staining of mouse liver using 28763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28763-1-AP (HRH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, Rattenhirngewebe
Erfolgreiche Detektion in IHCMauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28763-1-AP bindet in WB, IHC, ELISA HRH1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HRH1 fusion protein Ag30464
Vollständiger Name histamine receptor H1
Berechnetes Molekulargewicht 487 aa, 56 kDa
Beobachtetes Molekulargewicht56-60 kDa
GenBank-ZugangsnummerBC060802
Gene symbol HRH1
Gene ID (NCBI) 3269
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HRH1 antibody 28763-1-APProtokoll herunterladen
IHC protocol for HRH1 antibody 28763-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Wojciech (Verified Customer) (09-23-2025)

HRH1 was detected in T-cells producing ~60 kDa band with minimal background when tested by western blot at 1:1000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T-cells