HRPT2, CDC73 Monoklonaler Antikörper

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• KD/KO Validated

HRPT2, CDC73 Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B4H2

Kat-Nr. : 66490-1-PBS

Synonyme

CDC73, HRPT2, HRPT2; CDC73, 4B4H2, C1orf28

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  WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

  WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66490-1-Ig (same clone as 66490-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66490-1-Ig (same clone as 66490-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66490-1-PBS bindet in WB, IHC, IF-P, IF-Fro, ELISA HRPT2, CDC73 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HRPT2, CDC73 fusion protein Ag2959
• Vollständiger Name: cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 531 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC014351
• Gene symbol: HRPT2/CDC73
• Gene ID (NCBI): 79577
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).