HS2ST1 Polyklonaler Antikörper

HS2ST1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 12453-1-AP

Synonyme

2 O sulfotransferase, 2OST, HS2ST, HS2ST1, KIAA0448

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  WB analysis of human liver using 12453-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12453-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12453-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12453-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 12453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12453-1-AP (HS2ST1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, humanes Hirngewebe, humanes Herzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

12453-1-AP bindet in WB, IHC, ELISA HS2ST1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HS2ST1 fusion protein Ag3111
• Vollständiger Name: heparan sulfate 2-O-sulfotransferase 1
• Berechnetes Molekulargewicht: 229 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC025990
• Gene symbol: HS2ST1
• Gene ID (NCBI): 9653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Medicine (Baltimore); Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3; Authors - Guozhi Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bettina (Verified Customer) (08-05-2022)

Weak detection

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human pancreatic cancer cell line

HS2ST1 Antibody Western Blot validation (1:1000 dilution) in Human pancreatic cancer cell line (Cat no:12453-1-AP)