HSBP1 Polyklonaler Antikörper

HSBP1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10169-2-AP

Synonyme

HSBP1, HSF1BP

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10169-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10169-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10169-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10169-2-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10169-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10169-2-AP

  IP Result of anti-HSBP1 (IP:10169-2-AP, 4ug; Detection:10169-2-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10169-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10169-2-AP (HSBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 10169-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10169-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HT-1080.Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10169-2-AP bindet in WB, IP, IHC, ELISA HSBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSBP1 fusion protein Ag0225
• Vollständiger Name: heat shock factor binding protein 1
• Berechnetes Molekulargewicht: 8.5 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC007515
• Gene symbol: HSBP1
• Gene ID (NCBI): 3281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell Endocrinol; Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia; Authors - Li-Li Chen