HSD17B1 Polyklonaler Antikörper

HSD17B1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25334-1-AP

Synonyme

17 beta HSD 1, 20 alpha HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, HSD17, HSD17B1, SDR28C1

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 25334-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25334-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 25334-1-AP

  IP result of anti-HSD17B1 (IP:25334-1-AP, 4ug; Detection:25334-1-AP 1:500) with human placenta tissue lysate 2600 ug.

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  IHC staining of human placenta using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Endometriumkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25334-1-AP bindet in WB, IP, IHC, ELISA HSD17B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD17B1 fusion protein Ag17886
• Vollständiger Name: hydroxysteroid (17-beta) dehydrogenase 1
• Berechnetes Molekulargewicht: 328 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC104752
• Gene symbol: HSD17B1
• Gene ID (NCBI): 3292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Transl Med; An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer; Authors - Guan-Yu Ruan