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HSD17B12 Polyklonaler Antikörper

HSD17B12 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27461-1-PBS

Synonyme

17 beta HSD 12, 17-beta-HSD 12, 17-beta-hydroxysteroid dehydrogenase 12, 3 ketoacyl CoA reductase, 3-ketoacyl-CoA reductase

Filter:
  • Western Blot (WB) analysis of various lysates using HSD17B12 Polyclonal antibody (27461-1-AP)

    WB analysis using 27461-1-AP (same clone as 27461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using HSD17B12 Polyclonal antibody (27461-1-AP)

    IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using HSD17B12 Polyclonal antibody (27461-1-AP)

    IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSD17B12 Polyclonal antibody (27461-1-AP)

    IF Staining of HepG2 using 27461-1-AP (same clone as 27461-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSD17B12 antibody (27461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27461-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HSD17B12 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HSD17B12 fusion protein Ag26540
Vollständiger Name hydroxysteroid (17-beta) dehydrogenase 12
Berechnetes Molekulargewicht 312 aa, 34 kDa
Beobachtetes Molekulargewicht30 kDa
GenBank-ZugangsnummerBC012043
Gene symbol HSD17B12
Gene ID (NCBI) 51144
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).