HSD17B7 Polyklonaler Antikörper

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• KD/KO Validated

HSD17B7 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 14854-1-AP

Synonyme

17 beta HSD 7, 3 keto steroid reductase, HSD17B7, PRAP, SDR37C1

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  WB analysis of HEK-293 using 14854-1-AP

  WB result of HSD17B7 antibody (14854-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

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  WB analysis using 14854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14854-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14854-1-AP

  IP result of anti-HSD17B7 (IP:14854-1-AP, 4ug; Detection:14854-1-AP 1:1000) with MCF-7 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14854-1-AP bindet in WB, IHC, IF, IP, ELISA HSD17B7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD17B7 fusion protein Ag6639
• Vollständiger Name: hydroxysteroid (17-beta) dehydrogenase 7
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 32-38 kDa
• GenBank-Zugangsnummer: BC065246
• Gene symbol: HSD17B7
• Gene ID (NCBI): 51478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC	Reprod Biol Endocrinol; The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.; Authors - Xiaoyu Tu
mouse	IF	Cell Rep; Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.; Authors - Taha A Jan
human	WB	Nutrients; Malvidin-3-O-Glucoside Ameliorates Cadmium-Mediated Cell Dysfunction in the Estradiol Generation of Human Granulosa Cells; Authors - Shuer Liang
mouse	WB	Mol Hum Reprod; Semaphorin 4C regulates ovarian steroidogenesis through RHOA/ROCK1-mediated actin cytoskeleton rearrangement; Authors - Dan Chen
mouse	WB	Mol Cell Endocrinol; High concentrations of progesterone inhibit the expression of genes related to steroid metabolism in MA-10 Leydig cells; Authors - Liel-Sarah Izichkis