HSD3B1 Polyklonaler Antikörper

HSD3B1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32642-1-AP

Synonyme

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase

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  WB analysis using 32642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32642-1-AP (HSD3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32642-1-AP bindet in WB, ELISA HSD3B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD3B1 fusion protein Ag38103
• Vollständiger Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC031999
• Gene symbol: HSD3B1
• Gene ID (NCBI): 3283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD3B1, also known as hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, is an enzyme that plays a crucial role in steroid biosynthesis. It belongs to the short-chain dehydrogenase/reductase (SDR) family and is primarily involved in the conversion of C19 and C21 steroids with a 3β-hydroxy-5-ene structure to 3-oxo-4-ene products. This enzyme is mainly localized to the placenta and non-steroidogenic tissues. It is also involved in various biological processes, including C21-steroid hormone metabolic processes, hippocampus development, and response to corticosterone. HSD3B1 has been implicated in conditions such as hypertension and hypospadias. In addition, HSD3B1 has been studied in the context of prostate cancer, where it is involved in the synthesis of androgens within the tumor, contributing to resistance to radiotherapy and other treatments. Its expression and activity can be influenced by genetic variations, which may affect clinical outcomes in patients with prostate cancer. HSD3B1 is also involved in the metabolism of oxysterols, which are oxidized forms of cholesterol or its precursors. It catalyzes the oxidation of the 3β-hydroxy group to a 3-ketone and isomerizes the double bond from Δ5 to Δ4, a key reaction in bile acid biosynthesis. This enzyme's activity is essential for the conversion of initial 3β-hydroxy stereochemistry to the 3α-hydroxy stereochemistry in primary bile acids. HSD3B1 is also involved in the metabolism of side-chain oxysterols, which are ligands to various receptors and play roles in cholesterol biosynthesis and other biological processes. Overall, HSD3B1 is a multifunctional enzyme with significant roles in steroid hormone production, oxysterol metabolism, and various disease processes.