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HSD3B1 Polyklonaler Antikörper

HSD3B1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32642-1-PBS

Synonyme

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase

Filter:
  • Western Blot (WB) analysis of various lysates using HSD3B1 Polyclonal antibody (32642-1-AP)

    WB analysis using 32642-1-AP (same clone as 32642-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32642-1-AP (HSD3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32642-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

32642-1-PBS bindet in WB, Indirect ELISA HSD3B1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HSD3B1 fusion protein Ag38103
Vollständiger Name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Berechnetes Molekulargewicht 42 kDa
Beobachtetes Molekulargewicht42 kDa
GenBank-ZugangsnummerBC031999
Gene symbol HSD3B1
Gene ID (NCBI) 3283
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD3B1, also known as hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, is an enzyme that plays a crucial role in steroid biosynthesis. It belongs to the short-chain dehydrogenase/reductase (SDR) family and is primarily involved in the conversion of C19 and C21 steroids with a 3β-hydroxy-5-ene structure to 3-oxo-4-ene products. This enzyme is mainly localized to the placenta and non-steroidogenic tissues. It is also involved in various biological processes, including C21-steroid hormone metabolic processes, hippocampus development, and response to corticosterone. HSD3B1 has been implicated in conditions such as hypertension and hypospadias. In addition, HSD3B1 has been studied in the context of prostate cancer, where it is involved in the synthesis of androgens within the tumor, contributing to resistance to radiotherapy and other treatments. Its expression and activity can be influenced by genetic variations, which may affect clinical outcomes in patients with prostate cancer. HSD3B1 is also involved in the metabolism of oxysterols, which are oxidized forms of cholesterol or its precursors. It catalyzes the oxidation of the 3β-hydroxy group to a 3-ketone and isomerizes the double bond from Δ5 to Δ4, a key reaction in bile acid biosynthesis. This enzyme's activity is essential for the conversion of initial 3β-hydroxy stereochemistry to the 3α-hydroxy stereochemistry in primary bile acids. HSD3B1 is also involved in the metabolism of side-chain oxysterols, which are ligands to various receptors and play roles in cholesterol biosynthesis and other biological processes. Overall, HSD3B1 is a multifunctional enzyme with significant roles in steroid hormone production, oxysterol metabolism, and various disease processes.