HSD3B2 Monoklonaler Antikörper

HSD3B2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

2E7B4

Kat-Nr. : 67572-1-Ig

Synonyme

2E7B4, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3b HSD, 3-beta-HSD adrenal and gonadal type

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  WB analysis using 67572-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67572-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67572-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 67572-1-Ig

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 67572-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 67572-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HSD3B2 antibody (67572-1-Ig, Clone: 2E7B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Nebennierengewebe, Hausschwein-Nebennierengewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

67572-1-Ig bindet in WB, IHC, IF/ICC, ELISA HSD3B2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSD3B2 fusion protein Ag4291
• Vollständiger Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC038419
• Gene symbol: HSD3B2
• Gene ID (NCBI): 3284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in the placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

Publikationen

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	WB	Ecotoxicol Environ Saf; Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells; Authors - Pengchen Chen
human	WB	Birth Defects Res; Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells.; Authors - Junfeng Qi
human	WB	Gynecol Endocrinol; N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway; Authors - Yuan-Xue Jing
human	IHC	Pathol Res Pract; Disorganized adrenocortical zonational structure in COVID-19 patients: Implications of critical illness duration; Authors - Tian-Zi Wen
human	IHC,IF	Metabolism; Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19; Authors - Tian-Zi Wen