HSD3B7 Polyklonaler Antikörper

HSD3B7 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10488-1-AP

Synonyme

3 beta HSD VII, C(27) 3 beta HSD, HSD3B7, PFIC4, SDR11E3

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 10488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10488-1-AP (HSD3B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10488-1-AP (HSD3B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10488-1-AP

  IP result of anti-HSD3B7 (IP:10488-1-AP, 4ug; Detection:10488-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human pancreas cancer using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10488-1-AP bindet in WB, IP, IHC,ELISA HSD3B7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD3B7 fusion protein Ag0755
• Vollständiger Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004929
• Gene symbol: HSD3B7
• Gene ID (NCBI): 80270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD3B7(3 beta-hydroxysteroid dehydrogenase type 7) is also named as 3-beta-HSD VII,C(27) 3-beta-HSD and belongs to the 3-beta-HSD family. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids and HSD VII is active against four 7-alpha-hydroxylated sterols. This enzyme is expressed predominantly in the liver(PMID:11067870). Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1). It has 2 isoforms produced by alternative splicing.