HSDL1 Polyklonaler Antikörper

HSDL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16988-1-AP

Synonyme

HSDL1, SDR12C3

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• 

  WB analysis using 16988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 16988-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16988-1-AP bindet in WB, IHC, ELISA HSDL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSDL1 fusion protein Ag10588
• Vollständiger Name: hydroxysteroid dehydrogenase like 1
• Berechnetes Molekulargewicht: 330 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC018084
• Gene symbol: HSDL1
• Gene ID (NCBI): 83693
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy; Authors - Zhao-Xin Zhang
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico