HSF1 Polyklonaler Antikörper

HSF1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16107-1-PBS

Synonyme

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  WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 16107-1-AP (same clone as 16107-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16107-1-AP (same clone as 16107-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16107-1-AP (same clone as 16107-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16107-1-AP (same clone as 16107-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 16107-1-AP (same clone as 16107-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16107-1-PBS bindet in WB, IHC, Indirect ELISA HSF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSF1 fusion protein Ag9023
• Vollständiger Name: heat shock transcription factor 1
• Berechnetes Molekulargewicht: 529 aa, 57 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC014638
• Gene symbol: HSF1
• Gene ID (NCBI): 3297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).