HSF1 Monoklonaler Antikörper

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HSF1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F10A5

Kat-Nr. : 67189-1-PBS

Synonyme

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

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  WB analysis using 67189-1-Ig (same clone as 67189-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67189-1-Ig (HSF1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67189-1-Ig (same clone as 67189-1-PBS)

  WB result of HSF1 antibody (67189-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67189-1-Ig (same clone as 67189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67189-1-PBS bindet in WB, IHC, Indirect ELISA HSF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSF1 fusion protein Ag29061
• Vollständiger Name: heat shock transcription factor 1
• Berechnetes Molekulargewicht: 529 aa, 57 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC014638
• Gene symbol: HSF1
• Gene ID (NCBI): 3297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).