HSFY1 Polyklonaler Antikörper

HSFY1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11970-1-AP

Synonyme

HSF2 like, HSF2L, HSFY, HSFY1, HSFY2

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Galerie der Validierungsdaten

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  WB analysis of human testis using 11970-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11970-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11970-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11970-1-AP bindet in WB, IHC,ELISA HSFY1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSFY1 fusion protein Ag2592
• Vollständiger Name: heat shock transcription factor, Y-linked 1
• Berechnetes Molekulargewicht: 401 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC055414
• Gene symbol: HSFY1
• Gene ID (NCBI): 86614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.