HSL Polyklonaler Antikörper

HSL Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17333-1-PBS

Synonyme

LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79

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  WB analysis using 17333-1-AP (same clone as 17333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17333-1-AP (HSL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17333-1-PBS bindet in WB, IHC, Indirect ELISA HSL und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSL fusion protein Ag11360
• Vollständiger Name: lipase, hormone-sensitive
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC070041
• Gene symbol: HSL
• Gene ID (NCBI): 3991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.