HSP20 Monoklonaler Antikörper

HSP20 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7D2

Kat-Nr. : 67327-1-PBS

Synonyme

HspB6, Heat shock protein family B member 6, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock 20 kDa-like protein p20

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  WB analysis using 67327-1-Ig (same clone as 67327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67327-1-Ig (HSP20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67327-1-Ig (same clone as 67327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67327-1-Ig (HSP20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 67327-1-Ig (same clone as 67327-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  IF Staining of U-118 MG using 67327-1-Ig (same clone as 67327-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-118 MG cells using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50552-2

  Cytometric bead array standard curve of MP50552-2, HSPB6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67327-1-PBS. Detection antibody: 67327-4-PBS. Standard:Ag10484. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67327-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA HSP20 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP20 fusion protein Ag10484
• Vollständiger Name: heat shock protein, alpha-crystallin-related, B6
• Berechnetes Molekulargewicht: 160 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC068046
• Gene symbol: HSP20
• Gene ID (NCBI): 126393
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.