- Featured Product
- KD/KO Validated
HSP27 Monoklonaler Antikörper
HSP27 Monoklonal Antikörper für FC (Intra)
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human
Anwendung
FC (Intra)
Konjugation
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3E7A1
Kat-Nr. : CL647-66767
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL647-66767 bindet in FC (Intra) HSP27 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| Wirt / Isotyp | Maus / IgG1 | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | HSP27 fusion protein Ag27859 | 
| Vollständiger Name | heat shock 27kDa protein 1 | 
| Berechnetes Molekulargewicht | 19-23 kDa | 
| Beobachtetes Molekulargewicht | 27 kDa | 
| GenBank-Zugangsnummer | BC012768 | 
| Gene symbol | HSPB1 | 
| Gene ID (NCBI) | 3315 | 
| Konjugation | CoraLite® Plus 647 Fluorescent Dye | 
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm | 
| Form | Liquid | 
| Reinigungsmethode | Protein-G-Reinigung | 
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA | 
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| FC protocol for CL Plus 647 HSP27 antibody CL647-66767 | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
