HSP47/SERPINH1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HSP47/SERPINH1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2A8F6

Rezensionen (2)

Kat-Nr. : 67863-1-Ig

Synonyme

HSP47, SERPINH1, 2A8F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3

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  WB analysis using 67863-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67863-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67863-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67863-1-Ig

  WB result of HSP47/SERPINH1 antibody (67863-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells.

• 

  WB analysis of LNCaP using 67863-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67863-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 67863-1-Ig

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 67863-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 67863-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67863-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 67863-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, 4T1-Zellen, HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

67863-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA HSP47/SERPINH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP47/SERPINH1 fusion protein Ag30999
• Vollständiger Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC014623
• Gene symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC,IF,CoIP	iScience; SERPINH1 modulates apoptosis by inhibiting P62 ubiquitination degradation to promote bone metastasis of prostate cancer; Authors - Chen Tang ; KD Validated
rat	WB	J Proteome Res; Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts; Authors - Bridgette Hartley
human	WB,IP,CoIP	Elife; Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum; Authors - Ya-Juan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Donaldo (Verified Customer) (06-23-2025)

The antibody was very useful to bind/block HSP47 in human isolated platelets.

• Applications: Immunohistochemistry, Flow Cytometry

FH

Udesh (Verified Customer) (03-05-2024)

Worked well in WB in MSCs at 1:2000 in 5% milk

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs