HSP47/SERPINH1 Monoklonaler Antikörper

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• KD/KO Validated

HSP47/SERPINH1 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A8F6

Kat-Nr. : 67863-1-PBS

Synonyme

HSP47, SERPINH1, 2A8F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3

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  WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

  WB result of HSP47/SERPINH1 antibody (67863-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67863-1-Ig (same clone as 67863-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67863-1-Ig (same clone as 67863-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293T using 67863-1-Ig (same clone as 67863-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50781-1

  Cytometric bead array standard curve of MP50781-1, HSP47/SERPINH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67863-2-PBS. Detection antibody: 67863-1-PBS. Standard:Ag30999. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67863-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA HSP47/SERPINH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP47/SERPINH1 fusion protein Ag30999
• Vollständiger Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC014623
• Gene symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.