HSP60 Monoklonaler Antikörper

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HSP60 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F10E7

Kat-Nr. : 66041-1-PBS

Synonyme

HSPD1, 2F10E7, 60 kDa chaperonin, Chaperonin 60, CPN60

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  WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

  IP result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66041-1-Ig (same clone as 66041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50644-1

  Cytometric bead array standard curve of MP50644-1, HSPD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66041-1-PBS. Detection antibody: 66041-2-PBS. Standard:Ag7848. Range: 1.563-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66041-1-PBS bindet in WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA HSP60 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP60 fusion protein Ag7848
• Vollständiger Name: heat shock 60kDa protein 1 (chaperonin)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC003030
• Gene symbol: HSP60
• Gene ID (NCBI): 3329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)