HSP60 Monoklonaler Antikörper

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• KD/KO Validated

HSP60 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra), RIP

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

2F10E7

Kat-Nr. : CL488-66041

Synonyme

HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60

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  IF Staining of HepG2 using CL488-66041

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66041 (HSP60 antibody) at dilution of 1:50.

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  FC experiment of HepG2 using CL488-66041

  1X10^6 HepG2 cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human HSP60 (CL488-66041, Clone:2F10E7) (red), or 1 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 1.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• RIP: See 1 publications below

Produktinformation

CL488-66041 bindet in IF/ICC, FC (Intra), RIP HSP60 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP60 fusion protein Ag7848
• Vollständiger Name: heat shock 60kDa protein 1 (chaperonin)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC003030
• Gene symbol: HSP60
• Gene ID (NCBI): 3329
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Publikationen

Species	Application	Title
human	RIP	Cancer Lett; circCCNY enhances lenvatinib sensitivity and suppresses immune evasion in hepatocellular carcinoma by serving as a scaffold for SMURF1 mediated HSP60 degradation; Authors - Lei Yang