HSP70 Rekombinanter Antikörper

HSP70 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hefe, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4E10

Kat-Nr. : 80735-1-PBS

Synonyme

HSPA1A, 4E10, Heat shock 70 kDa protein 1A, heat shock 70kDa protein 1A, Heat shock protein family A member 1A

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  WB analysis using 80735-1-RR (same clone as 80735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80735-1-RR (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80735-1-RR (same clone as 80735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80735-1-RR (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80735-1-RR (same clone as 80735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung squamous cell carcinoma using 80735-1-RR (same clone as 80735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80735-1-RR (same clone as 80735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80735-1-RR (same clone as 80735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80735-1-RR (same clone as 80735-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP70 antibody (80735-1-RR, Clone: 4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80735-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80735-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HSP70 und zeigt Reaktivität mit Hefe, human, Maus, Ratten

• Getestete Reaktivität: Hefe, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HSP70 fusion protein Ag1446
• Vollständiger Name: heat shock 70kDa protein 1A
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC009322
• Gene symbol: HSP70
• Gene ID (NCBI): 3303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker