HSP90 Monoklonaler Antikörper

HSP90 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, CoIP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(103)

CloneNo.

3F11C1

Rezensionen (3)

Kat-Nr. : 60318-1-Ig

Synonyme

HSP90AA1, 3F11C1, EC:3.6.4.10, Heat shock protein family C member 1, Heat shock protein HSP 90 alpha

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  WB analysis using 60318-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  IP experiment of HepG2 using 60318-1-Ig

  IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug.

• 

  IHC staining of human ovary tumor using 60318-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60318-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60318-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  CoIP experiment of mouse brain using 60318-1-Ig

  Co-IP result of anti-HSP90 (IP:60318-1-Ig, 4ug; Detection:13422-1-AP 1:5000) with mouse brain tissue lysate 2240ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, ROS1728-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in CoIP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• COImmunpräzipitation (IP): COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 97 publications below
• IHC: See 4 publications below
• IF: See 11 publications below
• IP: See 6 publications below
• CoIP: See 5 publications below
• RIP: See 1 publications below

Produktinformation

60318-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA HSP90 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP90 fusion protein Ag3826
• Vollständiger Name: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Berechnetes Molekulargewicht: 853 aa, 90 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC023006
• Gene symbol: HSP90
• Gene ID (NCBI): 3320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application. This antibody may cross-react with HSP90AB1 due to the high homology between them.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
mouse,human	WB	Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu
rat	IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	WB	Aging Cell; Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis.; Authors - Davide D'Amico
human	WB,RIP,IP	Mol Cancer; Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.; Authors - Mengyan Xie
mouse,human	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiao (Verified Customer) (06-03-2024)

Works well in many cell types

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HT1080 and HEK293T

FH

Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 90 kd, as shown in the website.

• Applications: Western Blot

FH

Ayyappan (Verified Customer) (01-16-2019)

This antibody works well for IP in human cancer cell lines

• Applications: Immunoprecipitation,