HSP90 Monoklonaler Antikörper

HSP90 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, CoIP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F11C1

Kat-Nr. : 60318-1-PBS

Synonyme

HSP90AA1, 3F11C1, EC:3.6.4.10, Heat shock protein family C member 1, Heat shock protein HSP 90 alpha

---

• 

  WB analysis using 60318-1-Ig (same clone as 60318-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

  IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

• 

  CoIP experiment of mouse brain using 60318-1-Ig (same clone as 60318-1-PBS)

  Co-IP result of anti-HSP90 (IP:60318-1-Ig, 4ug; Detection:13422-1-AP 1:5000) with mouse brain tissue lysate 2240ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

60318-1-PBS bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA HSP90 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSP90 fusion protein Ag3826
• Vollständiger Name: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Berechnetes Molekulargewicht: 853 aa, 90 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC023006
• Gene symbol: HSP90
• Gene ID (NCBI): 3320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application. This antibody may cross-react with HSP90AB1 due to the high homology between them.