GRP94 Polyklonaler Antikörper

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• KD/KO Validated

GRP94 Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 10979-1-AP

Synonyme

HSP90B1, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, GP96

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  WB analysis using 10979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10979-1-AP (GRP94 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10979-1-AP

  WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells..

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  WB analysis of MCF-7 using 10979-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10979-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10979-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10979-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10979-1-AP

  IP result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

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  IHC staining of human breast cancer using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10979-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of human appendicitis using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 10979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 10979-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug GRP94 Polyclonal antibody (10979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe, humanes Appendizitis-Gewebe
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10979-1-AP bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA GRP94 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag1439
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC009195
• Gene symbol: GRP94
• Gene ID (NCBI): 7184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Publikationen

Species	Application	Title
mouse	WB	J Neuroinflammation; TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.; Authors - Na Liu
pig,human	WB	Vet Microbiol; ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.; Authors - Liang Zhang
rat	WB	Mol Med Rep; Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.; Authors - Maojun Liu
mouse	WB,IHC,IF	J Immunol Res; Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis; Authors - Qiaohui Hong
human	WB	Carcinogenesis; Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis; Authors - Huajie Zong
human	WB	Exp Brain Res; CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis; Authors - Danyang Wang