GRP94 Monoklonaler Antikörper

GRP94 Monoklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1H10B7

Kat-Nr. : 60012-1-Ig

Synonyme

ECGP, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

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Galerie der Validierungsdaten

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  WB analysis of human heart using 60012-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 60012-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 60012-1-Ig

  human lung tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60012-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60012-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60012-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60012-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60012-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 60012-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of HeLa using 60012-1-Ig

  Immunofluorescent analysis of Hela cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of MCF-7 using 60012-1-Ig

  Immunofluorescent analysis of MCF-7 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  FC experiment of HeLa using 60012-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug GRP94 antibody (60012-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human heart, humanes Herzgewebe, humanes Lebergewebe, humanes Lungengewebe, NIH/3T3-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

60012-1-Ig bindet in WB, IHC, IF, FC, CoIP, ELISA GRP94 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRP94 fusion protein Ag1439
• Vollständiger Name: heat shock protein 90kDa beta (Grp94), member 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC009195
• Gene symbol: HSP90B1
• Gene ID (NCBI): 7184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Publikationen

Species	Application	Title
human	WB	Autophagy; Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production.; Authors - Ruifang Wang
human	WB	Front Cell Dev Biol; Extracellular Vesicles Carry lncRNA SNHG16 to Promote Metastasis of Breast Cancer Cells via the miR-892b/PPAPDC1A Axis.; Authors - Wenfei Xia
rat	CoIP	Neurochem Res; Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1.; Authors - Jiajia Chen
human	WB	J Adv Res; Development of a novel thymidylate synthase (TS) inhibitor capable of up-regulating P53 expression and inhibiting angiogenesis in NSCLC.; Authors - Xin-Yang Li
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
		Front Physiol; Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.; Authors - Raul Chavez-Valdez