HSPA1B Rekombinanter Antikörper

HSPA1B Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242371E8

Kat-Nr. : 84873-4-RR

Synonyme

242371E8, HSP72

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  WB analysis using 84873-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84873-4-RR (HSPA1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84873-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84873-4-RR against Human HSPA1B were performed. The affinity constant is 9.62 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84873-4-RR bindet in WB, ELISA HSPA1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HSPA1B fusion protein Ag13700
• Vollständiger Name: heat shock 70kDa protein 1B
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC057397
• Gene symbol: HSP70
• Gene ID (NCBI): 3304
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSP70 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSP70 is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Recently it has been reported that HSP70 can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker.