HSPA1L Monoklonaler Antikörper

HSPA1L Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E7H2

Kat-Nr. : 66780-1-PBS

Synonyme

3E7H2, Heat shock 70 kDa protein 1-Hom, Heat shock 70 kDa protein 1-like, Heat shock protein family A member 1L, HSP70 1L

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  WB analysis of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66780-1-Ig (same clone as 66780-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66780-1-Ig (same clone as 66780-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66780-1-Ig (same clone as 66780-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66780-1-Ig (same clone as 66780-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66780-1-Ig (same clone as 66780-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66780-1-Ig (same clone as 66780-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSPA1L antibody (66780-1-Ig, Clone: 3E7H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66780-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HSPA1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSPA1L fusion protein Ag5182
• Vollständiger Name: heat shock 70kDa protein 1-like
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC034483
• Gene symbol: HSPA1L
• Gene ID (NCBI): 3305
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)